Solvent and Polarity Effects in Thin Layer Chromatography Lab Report

Solvent and Polarity Effects in clear Layer Chromatography - Lab Report ExampleThe adsorption chromatography is the most common type of TLC that analysts wont in the labs to carry out their experiments from the many types of TLCs that exist (Snyder 57). Solvents with low volatility and with low viscosities are broadly speaking used in the TLC experiment. Some of the commonly used solvents in the TLC process take hexane, ethanol, pentane, hydrocarbon mixture, acetone, ethyl acetate and diethyl ether because they are much cheaper and due to the fact that they are non-toxic. The distance that a compound travels on the silicon plate will depend largely on the compounds affinity for the absorption material (Silicon plate) in comparison with the eluent (the solvent in use). This experiment determines the effect of signaling on elution as well as determines the TLC of an unknown organic compound.120 mL jars, puree papers, hexane, ethyl acetate, acetone, ethanol, measuring cylinder, si lica gel, TLC plates, lead pencils, trans-stilbene, 9-flourenone, benzoic acid, methylyene chloride, spotter and Iodine.5. In a expose set up, dissolve in a test tube 2 mg of benzoic acid, 9-fluorenone and trans-stilbene in 10 drops of methylene chloride. Label each test tube. learn the observations in each test tube.6. intent a spotter to apply a puny amount of trans-stilbene at the first point on the first line on one plate very briefly using a spotter. Here, the resolving should move by capillary action. Similarly, apply some amount of benzoic acid and 9-fluorenone on the minute of arc and the third line respectively. Look at the spots under ultraviolet light to see clearly. NB It is important to protect ones eyes using a protective device worry a goggle to avoid eye contamination from the dangerous ultraviolet rays. Add some little solvent in each line of the plate again if the lines are not clearly visible. Record